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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肾连蛋白(NPNT)是一种蛋白质,是由 NPNT 基因编码的。该基因能使整合素结合活性。参与几个过程,包括由整合素介导的细胞-细胞粘附;ERK1和 ERK2 级联的正向调节;和成骨细胞分化的正向调节。其作用于转化生长因子β受体信号通路的上游或正向调节。位于细胞外体中。是含胶原蛋白的细胞外基质的一部分。与 NPNT 相关的疾病包括肾病综合征,5 型,伴有或不伴有眼部异常和纤维肉瘤性骨肉瘤。其相关途径有 MET 促进细胞运动。与该基因相关的基因本体论包括钙离子结合和整合素结合。该基因的一个重要同源物是 EGFL6。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肾连蛋白(NPNT)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肾连蛋白(NPNT)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human NPNT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human NPNT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.