BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肿瘤坏死因子-β（TNF-β）也称淋巴毒素-α（LT-α），是一种蛋白质，在人类中由LTA基因编码。属于造血细胞系，LT-α表现出抗增殖活性，导致肿瘤细胞系的细胞破坏。作为一种细胞毒性蛋白，它在免疫调节中发挥着各种重要作用，取决于它分泌的形式。与TNF超家族的其他成员不同，LT-α仅以可溶性同源三聚体的形式存在，当在细胞表面发现时，它仅以与LTβ的异源三聚体的形式存在。它被翻译成一个25 kDa的糖基化多肽，有171个氨基酸残基。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人肿瘤坏死因子β(TNF-β)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肿瘤坏死因子β(TNF-β)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TNF-β. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TNF-β present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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