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肿瘤坏死因子样配体1A(TL1A;TNFSF15),也被称为血管内皮生长抑制剂(VEGI)和TNF超家族成员15,是由TNFSF15基因编码的蛋白质。它是一种抗血管生成蛋白。它属于肿瘤坏死因子超家族,是第15个成员。它是死亡受体3的唯一已知配体。它也可以被诱饵受体3识别。这种蛋白质在内皮细胞中大量表达,但在B细胞或T细胞中均不表达。这种蛋白质的表达是由TNF-α和IL-1α诱导的。它可以激活NF-κB和MAPK信号通路,并作为自分泌因子诱导内皮细胞凋亡。这种细胞因子也被发现抑制内皮细胞增殖,因此可以作为血管生成抑制剂。已经报道了由替代剪接转录本变体编码的另一种亚型,但尚未确定该转录本的序列。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肿瘤坏死因子样配体1A(TL1A;TNFSF15)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肿瘤坏死因子样配体1A(TL1A;TNFSF15)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TL1A;TNFSF15. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TL1A;TNFSF15 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.