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LIGHT又称肿瘤坏死因子配体超家族成员14(TNFSF14),是TNF超家族的一种分泌蛋白。它被疱疹病毒进入媒介(HVEM)以及诱饵受体3识别。LIGHT代表 "与淋巴毒素同源,表现为诱导性表达,并与HSV糖蛋白D竞争与疱疹病毒进入介质结合,后者是T淋巴细胞上表达的受体"。在分化群术语中,它被归类为CD258。该基因所编码的蛋白是肿瘤坏死因子(TNF)配体家族的成员。该蛋白可能是激活淋巴细胞的一种刺激因子,也是对疱疹病毒感染的一种阻遏。该蛋白已被证明可以刺激T细胞的增殖,触发各种肿瘤细胞的凋亡,并在血管正常化过程中发挥作用。该蛋白还被报道可以防止肿瘤坏死因子α介导的原代肝细胞凋亡。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肿瘤坏死因子配体超家族成员14(TNFSF14)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肿瘤坏死因子配体超家族成员14(TNFSF14)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TNFSF14. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TNFSF14 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.