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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肿瘤易感基因101,也被称为TSG101,是是一种编码同名细胞蛋白的基因。该基因编码的蛋白质属于一组明显无活性的泛素偶联酶同系物。该基因产物包含一个盘绕的线圈结构域,与stathmin相互作用,stathmin是一种与肿瘤发生有关的胞质磷酸蛋白。该蛋白质可能在细胞生长和分化中发挥作用,并充当负生长调节剂。该肿瘤易感基因的体外稳态表达似乎对维持基因组稳定性和细胞周期调节很重要。该基因的突变和替代剪接在乳腺癌中发生频率很高,并表明缺陷发生在乳腺癌肿瘤发生和/或进展过程中。它的主要作用是参与ESCRT通路。该途径促进了反向拓扑萌芽和多泡体(MVB)的形成,其将预定降解的货物输送到溶酶体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肿瘤易感基因101(TSG101)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肿瘤易感基因101(TSG101)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TSG101. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TSG101 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.