计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胰岛素样生长因子(IGF)结合蛋白-3(IGFBP-3)是 IGFBP 家族的六个成员之一,是 IGF 通路中的一个关键蛋白。IGFBP-3 的功能既依赖于 IGF,也不依赖于 IGF。IGFBP-3 依赖于 IGF 的作用包括将 IGF 从合成部位输送到具有 IGF 受体的靶细胞并激活相关下游信号传导的内分泌作用。IGFBP-3 与 IGF 无关的作用包括与细胞外基质蛋白和质膜蛋白相互作用,通过质膜转运到细胞质和细胞核。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人胰岛素样生长因子3(IGF-3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人胰岛素样生长因子3(IGF-3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IGF-3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IGF-3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.