BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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胰岛素降解酶(IDE)是M16金属蛋白酶家族中的一种大型锌结合蛋白酶，已知可裂解多种序列差异很大的短多肽。这个家族的其他成员包括线粒体加工肽酶和前序列蛋白酶。IDE最早是通过降解激素胰岛素的B链的能力被确认的。这一活性在60多年前就被观察到了，尽管专门负责B链裂解的酶是最近才被鉴定出来的。这一发现揭示了IDE和之前被鉴定的细菌蛋白酶pitrilysin之间有相当大的氨基酸序列相似性，表明有共同的蛋白酶解机制。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人胰岛素降解酶(IDE)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人胰岛素降解酶(IDE)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IDE. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IDE present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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