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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Caspase-1/白细胞介素-1转换酶（ICE）是一种进化保守的酶，它能将其他蛋白质，如炎症细胞因子白细胞介素1β和白细胞介素18的前体以及热病诱导剂Gasdermin D，蛋白分解为活性成熟肽。一旦通过形成炎症体复合物被激活，它就会通过裂解启动两种炎症细胞因子--白细胞介素1β（IL-1β）和白细胞介素18（IL-18），以及通过裂解Gasdermin D启动火毒症（一种程序性细胞死亡途径）被Caspase-1激活的两种炎症细胞因子会从细胞中排出，进一步诱发邻近细胞的炎症反应。与CASP1相关的疾病包括牛痘和志贺氏病。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人胱天蛋白酶1(CASP1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人胱天蛋白酶1(CASP1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CASP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CASP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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