BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
脱嘌呤脱嘧啶核酸内切酶1(APE1)是一种酶，是由APEX1基因编码的。Apurinic/apyrimidinic（AP）位点（也称为 "消减位点"）通过自发水解、DNA损伤剂或去除特定异常碱基的DNA糖基化酶在DNA分子中经常发生。AP位点是突变前的病变，可以阻止正常的DNA复制。所有细胞，都进化出了识别和修复这种位点的系统。第二类AP内切酶在AP位点的5'处裂解磷酸二酯骨架，从而启动了一个被称为碱基切除修复（BER）的过程。APEX基因（备选名称为APE1、HAP1、APEN）编码了细胞中的主要AP内切酶。已发现该基因的剪接变体；所有剪接变体都编码相同的蛋白质。它已被证明与MUTYH、瓣膜结构特异性内切酶1和XRCC1相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人脱嘌呤脱嘧啶核酸内切酶1(APE1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人脱嘌呤脱嘧啶核酸内切酶1(APE1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human APE1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human APE1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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