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脱氧胞苷三磷酸焦磷酸酶1(DCTPP1),也被称为dCTP焦磷酸酶1,是由DCTPP1基因编码的酶。该基因编码的蛋白质是dCTP焦磷酸酶,它将dCTP转化为dCMP和无机焦磷酸盐。编码的蛋白质对dTTP和dATP也表现出弱活性,但对dGTP没有。这种蛋白质可能负责在DNA合成后消除过量的dCTP,并可能防止CpG岛的过度甲基化。已经为该基因发现了三种转录本变体,一种蛋白质编码和另外两种非蛋白质编码。与它相关的疾病包括天冬氨酸氨基糖尿症和耳硬化症。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人脱氧胞苷三磷酸焦磷酸酶1(DCTPP1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人脱氧胞苷三磷酸焦磷酸酶1(DCTPP1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human DCTPP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human DCTPP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.