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腺苷酸环化酶相关蛋白1（CAP1）是一种多功能蛋白，由CAP1基因编码，与酿酒酵母CAP蛋白有关，参与环状AMP通路。CAP1在细胞内具有六个结构域，能够调控细胞肌动蛋白骨架解聚、Cofilin活性等。近年来研究发现，CAP1在多种恶性肿瘤中过表达，与癌细胞的增殖、迁移和侵袭密切相关，是临床恶性肿瘤诊断、治疗和预后的重要研究靶点。此外，CAP1还与炎症、代谢综合征等疾病相关，如通过与抵抗素结合影响炎症信号通路。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人腺苷酸环化酶相关蛋白1(CAP1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人腺苷酸环化酶相关蛋白1(CAP1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CAP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CAP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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