计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
色氨酸-2,3-双加氧酶(TDO),也被称为色氨酸酶(TO)或TDO2,是由TDO2基因编码的酶。在酶学中,它是一种血红素酶,其催化L-色氨酸氧化成N-甲酰基-L-犬尿氨酸,这是犬尿氨酸途径的第一个限速步骤。它作为色氨酸代谢整个生物学过程的一部分,在色氨酸通量的生理调节中起着重要作用。它催化色氨酸降解的第一步和限速步骤沿着犬尿氨酸途径, 从而调节全身色氨酸水平。这种酶属于氧化还原酶家族,特别是那些以O2作为氧化剂作用于单一供体并将两个氧原子掺入底物(加氧酶)中的酶。该家族包括TDO和密切相关的吲哚胺 2,3-双加氧酶 (IDO)。TDO和IDO每个单体都含有一种非共价结合的血红素;TDO通常是四聚体,而IDO是单体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人色氨酸-2,3-双加氧酶(TDO)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人色氨酸-2,3-双加氧酶(TDO)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TDO. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TDO present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.