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有丝分裂检查点丝氨酸/苏氨酸蛋白激酶 BUB1 也称为 BUB1,是由 BUB1 基 因编码的酶。Bub1 是在酿酒酵母(Saccharomyces cerevisiae)基因筛查 中首次发现的丝氨酸/苏氨酸蛋白激酶。与 BUB1 相关的疾病包括结直肠癌和 镶嵌式变异非整倍体综合征。它的相关途径包括姐妹染色体的分离和细胞周 期_APC 在细胞周期调节中的作用。与该基因相关的基因本体论注释包括转 移酶活性、转移含磷基团和蛋白丝氨酸/苏氨酸激酶活性。该基因的一个重要 类似物是 BUB1B。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人苏氨酸蛋白激酶BUB1(BUB1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人苏氨酸蛋白激酶BUB1(BUB1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human BUB1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human BUB1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.