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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蛋白精氨酸脱亚氨酶4，也被称为PADI4，是由PADI4基因编码的蛋白质。该基因是基因家族的成员，该基因家族编码负责将精氨酸转化为瓜氨酸残基的酶。该基因可能在粒细胞和巨噬细胞发育中发挥作用，导致炎症和免疫反应。它在表观遗传学中起作用，组蛋白3和4上的精氨酸的脱氨可以拮抗精氨酸甲基化。该蛋白质可能存在于低聚物中，并结合每个亚基的5个钙离子。它通常存在于细胞质、细胞核和嗜酸性粒细胞和嗜中性粒细胞中。它在外周单核细胞或淋巴细胞中不表达 。它也在类风湿性关节炎滑膜组织中表达。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人蛋白精氨酸脱亚氨酶4(PADI4)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人蛋白精氨酸脱亚氨酶4(PADI4)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PADI4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PADI4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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