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血管内皮钙黏蛋白5(Cadherin 5),2型或VE-cadherin(血管内皮黏蛋白)也被称为CD144(分化簇144),是一种黏蛋白。它是由基因CDH5编码的。VE-cadherin是黏蛋白超家族中的一种经典黏蛋白,该基因位于16号染色体长臂上的一个六黏蛋白簇中,该区域涉及乳腺癌和前列腺癌的异质性丢失事件。编码的蛋白是一种钙依赖性的细胞-细胞粘附糖蛋白,由五个细胞外黏蛋白重复序列、一个跨膜区和一个高度保守的细胞质尾部组成。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人血管内皮钙黏蛋白5(CDH5)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人血管内皮钙黏蛋白5(CDH5)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CDH5. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CDH5 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.