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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血红蛋白(或血红蛋白;Hpx;Hx),又称β-1B-糖蛋白,是一种糖蛋白,在人类中由HPX基因编码,属于血红蛋白家族的蛋白。单独在血浆中循环的血红蛋白(称为游离血红蛋白,而不是与红细胞一起生活和循环的血红蛋白)很快就会被氧化成高铁血红蛋白,然后与球蛋白链一起进一步解离成游离血红蛋白。然后,游离血红素将被氧化成游离甲状腺素,血红蛋白迟早会与游离甲状腺素结合在一起,形成甲状腺素和血红蛋白的复合物,继续它们在循环中的旅程,直到到达脾脏、肝脏和骨髓中的肝细胞或巨噬细胞的受体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人血红素结合蛋白(HPX)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人血红素结合蛋白(HPX)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human HPX. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human HPX present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.