BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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C3转化酶（C4bC2b，以前叫C4b2a）属于丝氨酸蛋白酶家族，作为补体系统的一部分，在先天免疫中是必要的，补体系统最终会使颗粒蛋白化，释放炎症肽，形成C5转化酶和细胞裂解。C3转化酶可以用来指替代途径（C3bBb）或经典和凝集素途径（C4bC2b，以前是C4b2a）中产生的形式。一旦形成，两种C3转化酶都会催化C3的蛋白水解裂解为C3a和C3b（因此被称为"C3转化酶"）。被称为C3a的较小片段的作用是增加血管通透性，促进吞噬细胞的外渗，而较大的C3b片段可作为Opsonin或与任一类型的C3转化酶结合，形成三分子C5转化酶以激活C5用于膜攻击复合物。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人补体C3转化酶(C3 Convertase)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人补体C3转化酶(C3 Convertase)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human C3 Convertase. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human C3 Convertase present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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