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C5转化酶是一种属于丝氨酸蛋白酶家族的酶，在先天免疫中起着关键作用。它参与补体系统，最终导致细胞死亡。有四种不同的C5转化酶能够专门将蛋白质C5转化为C5a和C5b片段。其中两种转化酶是生理性的补体酶，与细胞表面结合，介导补体系统的经典途径（C4b2b3b，或C4b2a3b）或替代途径（C3bBbC3b）。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人补体C5转化酶(C5c)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人补体C5转化酶(C5c)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human C5c. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human C5c present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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