BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
补体成分5是一种蛋白质，是由C5基因编码的。补体成分5参与补体系统。它被裂解为C5a和C5b。补体成分5是补体的第五种成分，在炎症和细胞杀伤过程中起着重要作用。该蛋白由α和β多肽链组成，通过一个二硫桥连接。一种激活肽，即C5a，是一种具有强大的致痉挛和趋化活性的无细胞毒素，通过与C5转化酶的裂解从α多肽中得到。C5b大分子裂解产物可与C6补体成分形成复合物，这种复合物是形成膜攻击复合物的基础，其中包括其他补体成分。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人补体成分5(C5)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人补体成分5(C5)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human C5. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human C5 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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