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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
趋化因子C-C-基元受体7(CCR7)是一种蛋白质,由CCR7基因编码。该受体的两个配体已被确认:趋化因子(C-C图案)配体19(CCL19/ELC)和(C-C图案)配体21(CCL21)。它最近也被指定为CD197(分化群197)。该基因所编码的蛋白是G蛋白偶联受体家族的成员。该受体被确定为由爱泼斯坦-巴尔病毒(EBV)诱导的基因,并被认为是EBV对B淋巴细胞影响的媒介。这种受体在各种淋巴组织中表达,并激活B淋巴细胞和T淋巴细胞。它已被证明可刺激树突状细胞的成熟。它还参与了T细胞对各种次级淋巴器官(如淋巴结和脾脏)的归巢,以及T细胞在脾脏内的贩运。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人趋化因子C-C-基元受体7(CCR7)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人趋化因子C-C-基元受体7(CCR7)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CCR7. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CCR7 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.