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趋化因子C-C-基元配体16(CCL16)是一种属于CC趋化因子家族的小细胞因子，包括肝脏表达的趋化因子（LEC）和单抗素-1（MTN-1）。这种趋化因子由肝脏、胸腺和脾脏表达，对单核细胞和淋巴细胞有螯合作用。CCL16的细胞表达可由IL-10、IFN-γ和细菌脂多糖在单核细胞中强烈诱导。它的基因位于17号染色体上，属于其他CC趋化因子的集群。它通过与细胞表面的趋化因子受体如CCR1、CCR2、CCR5和CCR8相互作用对细胞产生影响。它已被证明可抑制骨髓祖细胞的快速增殖。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人趋化因子C-C-基元配体16(CCL16)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人趋化因子C-C-基元配体16(CCL16)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CCL16. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CCL16 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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