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跨膜蛋白9（TMEM9）是一种由TMEM9基因编码的蛋白质，位于染色体1q41。它编码的蛋白质由183个氨基酸组成，其N端区域包含多个重要结构域。TMEM9在细胞内运输等过程中可能发挥作用，并参与了多种癌症的发展，如结直肠癌、肝癌和乳腺癌等。研究发现，TMEM9在多种癌症中表现出异常表达，对患者的生存预后有显著影响。它可能通过调节关键致癌途径，如Wnt信号通路，参与肿瘤的发生和发展。此外，TMEM9还与免疫浸润、肿瘤突变负荷和微卫星不稳定性相关，表明其在肿瘤微环境和基因组稳定性方面发挥着重要作用。这些发现突出了TMEM9作为多种癌症预后生物标志物和治疗靶点的潜力。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人跨膜蛋白9(TMEM9)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人跨膜蛋白9(TMEM9)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TMEM9. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TMEM9 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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