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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转录中介因子1γ(TIF1g),也被称为含有33的三方基序(TRIM33),由TRIM33基因编码。该基因编码的蛋白质被认为是转录辅助抑制因子。但是,与相关的TIF1-α和TIF1-β蛋白不同,很少有转录因子(如Smad4)与TIF1-γ相互作用。该蛋白质是三方基序家族的成员。该基序包括三个锌结合结构域:指环、B-box 1型锌指、B-box 2型锌指和一个盘绕线圈区域。该基因的三个交替剪接的转录本变体已被描述,但是,一个变体的全长性质尚未确定。它已被证明可以与TRIM24相互作用。它作为肿瘤抑制基因,预防慢性粒单核细胞白血病的发展。它还调节TGF-β1受体,促进造血干细胞的生理衰老。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人转录中介因子1γ(TIF1g)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人转录中介因子1γ(TIF1g)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TIF1g. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TIF1g present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.