BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转录调节蛋白(BACH2)是一种由 BACH2 基因编码的蛋白。它的 N 端含有一 个 BTB/POZ 结构域，形成一个二硫连接的二聚体，C 端有一个 bZip_Maf 结构域。在 T 细胞中，BACH2 在体外和体内（如在银屑病皮肤中）都被转 录因子维生素 D 受体（VDR）招募，是维生素 D 在这些细胞中调节功能的 重要组成部分。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人转录调节蛋白BACH2(BACH2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人转录调节蛋白BACH2(BACH2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human BACH2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human BACH2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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