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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
辅肌动蛋白α2(α-actinin-2)是一种蛋白质,由 ACTN2 基因编码。该基因 编码的α-actinin 异构体在骨骼肌和心肌中均有表达,其功能是将肌纤维肌动 蛋白细丝和肌球蛋白固定在 Z-盘上。辅肌动蛋白α2 是一个 103.8 kDa 的蛋 白质,由 894 个氨基酸组成。每个分子都是杆状的(长度为 35 纳米),它 以反平行的方式进行同源二聚。每个单体都有一个 N 端肌动蛋白结合区,由 两个 Calponin 同源结构域组成,两个 C 端 EF 手结构域,四个串联的 spectrin 样重复物在分子的中心区域形成杆状结构域。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人辅肌动蛋白α2(ACTN2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人辅肌动蛋白α2(ACTN2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ACTN2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ACTN2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.