BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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过氧化还原酶3(PRDX3)是由PRDX3基因编码的，该基因编码是一种具有抗氧化功能的蛋白质，定位于线粒体。该基因与编码鼠疫沙门氏菌烷基过氧化氢还原酶C22亚单位的基因显示出明显的核苷酸序列相似性。该基因产物在缺乏C22亚基基因的大肠杆菌中的表达可以挽救该细菌对烷基过氧化氢的抗性。已发现该基因有两个转录变体，编码两种不同的异构体。它已被证明与MAP3K13相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人过氧化还原酶3(PRDX3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人过氧化还原酶3(PRDX3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PRDX3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PRDX3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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