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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
运动因子相关蛋白1(MRP1)是一个编码蛋白质的基因,它是跨膜4超家族的成员,也被称为四链蛋白家族。它是一种细胞表面糖蛋白,由四个跨膜区组成,有两个细胞外环,含有二硫键,这在整个四板蛋白家族中是保守的。还含有明显的棕榈酰化位点,使它能与脂质和其他蛋白相互作用。它还可以调节细胞粘附和迁移。在某些类型的黑色素瘤、乳腺癌、肺癌、胰腺癌和结肠癌中,它的过度表达被证明可以减少转移。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人运动因子相关蛋白1(MRP1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人运动因子相关蛋白1(MRP1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MRP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MRP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.