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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
金属硫蛋白是一个富含半胱氨酸、低分子量(MW从500到14000Da)的蛋白质家族。它们定位在高尔基体的膜上。MTs有能力通过其半胱氨酸残基的硫醇基团结合生理性(如锌、铜、硒)和异生物(如镉、汞、银、砷)重金属,这占其组成氨基酸残基的近30%。在人类中主要有四种异构体表达:MT1(亚型A、B、E、F、G、H、L、M、X),MT2,MT3,和MT4。在人体内,主要在肝脏和肾脏中大量合成。它们的产生取决于膳食中的矿物质,如锌、铜和硒,以及氨基酸组氨酸和半胱氨酸的可用性。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人金属硫蛋白1(MT-1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人金属硫蛋白1(MT-1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MT-1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MT-1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.