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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
溶质载体家族30成员8(SLC30A8),也被称为锌转运蛋白8(ZNT8),是由SLC30A8基因编码的蛋白质。它是与胰岛素分泌相关的锌转运蛋白。该基因的某些等位基因可能会增加患2型糖尿病的风险,但功能丧失突变似乎会大大降低患糖尿病的风险。该基因编码的蛋白质是参与细胞内囊泡中锌积累的锌外排转运蛋白。该基因仅在胰腺中高水平表达,特别是在朗格汉斯岛中。编码的蛋白质在分泌胰岛素的INS-1细胞的分泌途径颗粒中与胰岛素共定位。该基因的等位基因变异存在,赋予糖尿病易感性,非胰岛素依赖性(NIDDM)。已经为该基因发现了几种编码不同亚型的转录本变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人锌转运体8(ZNT8)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人锌转运体8(ZNT8)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ZNT8. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ZNT8 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.