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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
METRNL(Meteorin Like, Glial Cell Differentiation Regulator)也被称为 subfatin 和 cometin,是一种小的(约 27kDa)分泌性细胞因子,由一个叫 做 meteorin-like(METRNL)基因编码的蛋白质。它在粘膜组织、皮肤和 活化的巨噬细胞中高度表达。Metrnl 也被描述为一种激素。对人类皮肤相关 疾病的筛选显示,METRNL 在银屑病、结节性瘙痒症、光化性角化病和特应 性皮炎中明显过度表达。METRNL 在人类类风湿关节炎的滑膜中也是上调 的。脂肪细胞 Metrnl 通过改善脂肪功能,包括脂肪细胞分化、新陈代谢激 活和炎症抑制,拮抗肥胖引起的胰岛素抵抗血清中 Metrnl 水平较低可能是 患冠心病和 2 型糖尿病的风险因素。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人镍纹蛋白样蛋白(METRNL)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人镍纹蛋白样蛋白(METRNL)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human METRNL. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human METRNL present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.