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长寿保证同源物2(LASS2),也被称为神经酰胺合成酶2(CERS2)或肿瘤转移抑制基因1蛋白(TMSG1),是由CERS2基因编码的酶。它是一种神经酰胺合酶,可催化合成非常长的酰基链神经酰胺,包括C20和C26神经酰胺。它是所有CerS中最普遍表达的,并且分布最广。它于2001年首次被发现。它包含几乎所有CerS共有的保守TLC域和类似Hox的域。它的表达在活动性髓鞘形成期间短暂增加,表明它对髓鞘脂的合成很重要。它基因大小紧凑,位于细胞周期早期复制的染色体区域 。它的活性由鞘氨醇-1-磷酸(S1P)通过CerS2上两个独立运作的鞘氨醇-1-磷酸受体样残基进行调节。与正常组织相比,乳腺癌组织中的CerS2水平显着升高。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人长寿保证同源物2(LASS2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人长寿保证同源物2(LASS2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LASS2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LASS2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.