计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
高密度脂蛋白(HDL)是五大类脂蛋白之一。脂蛋白是由多种蛋白质组成的复杂颗粒,在细胞外的水中将所有脂肪分子(脂质)运送到身体周围。它们通常由每粒80-100个蛋白质组成(由一个、两个或三个载脂蛋白A组织。高密度脂蛋白颗粒在血液中循环时扩大,聚集更多的脂肪分子),每个颗粒可运输多达数百个脂肪分子。脂蛋白按密度/大小分为5个亚组(反比关系),这也与功能和心血管事件的发生率相关。与较大的脂蛋白颗粒将脂肪分子输送到细胞不同,高密度脂蛋白颗粒将脂肪分子从细胞中移除。携带的脂质包括胆固醇、磷脂和甘油三酯,每一种的数量都是可变的。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人高密度脂蛋白(HDL)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人高密度脂蛋白(HDL)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human HDL. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human HDL present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.