BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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高尔基体蛋白73（GP73，又称Golph2），也被称为高尔基磷蛋白-2，是一种常驻高尔基体的特异性膜蛋白，在正常肝脏中由胆道上皮细胞表达。它有400个氨基酸，是一种73 kDa的跨膜糖蛋白，通常存在于顺式高尔基复合物中。高尔基体主要参与蛋白质的分泌、糖基化和膜转化，细胞空间结构的维持和功能的执行与高尔基体的膜驻留蛋白密切相关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人高尔基蛋白73(GP73)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人高尔基蛋白73(GP73)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human GP73. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human GP73 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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