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分化簇80(也称CD80和B7-1)是免疫球蛋白超家族中的一种B7、I型膜蛋白,有一个细胞外免疫球蛋白恒定样结构域和一个受体结合所需的可变样结构域。它与另一个B7蛋白(B7-2)CD86密切相关,并经常串联起作用。CD80和CD86都与成本刺激受体CD28和CTLA-4(CD152)相互作用。CD80可以在各种免疫细胞的表面发现,包括B细胞、单核细胞或T细胞,但最典型的是在抗原呈递细胞(APC),如树突状细胞。CD80是T细胞表面发现的蛋白质CD28(用于自动调节和细胞间结合)和CTLA-4(用于调节的减弱和细胞解离)的配体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠 CD80(CD80)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠 CD80(CD80)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse CD80. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse CD80 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.