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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
60kD 热休克蛋白(HSP60),也称为伴侣蛋白( Cpn ),是一个热休克蛋白家族,最初按其 60kDa 的分子量分类。它们通过协助蛋白质折叠来防止蛋白质在高温等压力情况下的错误折叠。HSP60 属于协助蛋白质折叠的一大类分子,称为分子伴侣。新制造的蛋白质通常必须从氨基酸的线性链折叠成三维三级结构。折叠蛋白质的能量由每种蛋白质的氨基酸侧链之间的非共价相互作用和溶剂效应提供。大多数蛋白质自发折叠成最稳定的三维构象,这通常也是它们的功能构象,但偶尔蛋白质会错误折叠。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠热休克蛋白60(Hsp60)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠热休克蛋白60(Hsp60)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Hsp60. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Hsp60 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.