BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
衰变加速因子又称CD55或DAF，是一种蛋白质，它调节着细胞表面的补体系统。它能识别C4b和C3b片段，这些片段是在激活C4（经典或凝集素途径）或C3（替代途径）时产生的。它与经典和凝集素途径的细胞相关C4b相互作用，干扰C2向C2b的转化，从而防止C4b2a C3转化酶的形成，它与替代途径的C3b相互作用，干扰D因子将B因子转化为Bb，从而防止替代途径的C3bBb C3转化酶的形成。因此，通过限制补体级联的扩增转化酶，DAF间接地阻止了膜攻击复合物的形成。这种糖蛋白广泛分布于造血细胞和非造血细胞中。它是克罗默血型系统的一个决定因素。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人衰变加速因子(DAF;CD55)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人衰变加速因子(DAF;CD55)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human DAF;CD55. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human DAF;CD55 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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