计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
嗜酸性粒细胞过氧化物酶是一种存在于嗜酸性粒细胞、人类和哺乳动物的先天免疫细胞中的酶。这种氧化还原酶蛋白由基因EPX编码,在这些骨髓细胞中表达。它与其直系同源过氧化物酶、髓过氧化物酶(MPO)、乳过氧化物酶(LPO)和甲状腺过氧化物酶(TPO)有许多相似之处。蛋白质集中在嗜酸性粒细胞内的分泌颗粒中。它是一种血红素过氧化物酶,其活性包括将卤离子氧化成杀菌剂。活性氧、细菌细胞壁的阳离子破坏和蛋白质氨基酸残基的翻译后修饰。它的主要功能是催化溶液中的过氧化氢和卤离子形成次卤酸。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠嗜酸性粒细胞过氧化物酶(EPX)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠嗜酸性粒细胞过氧化物酶(EPX)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse EPX. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse EPX present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.