BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
伏马毒素(Fumonisins)是一组来自镰刀菌及其利塞拉科的霉菌毒素。它在结构上与鞘氨醇相似，鞘氨醇是细胞鞘脂的主要长链基础骨架，并已被证明是鞘氨醇和鞘氨醇N-酰基转移酶的竞争性抑制剂。伏马毒素对这种酶的抑制产生对鞘脂代谢的破坏，导致动物血清和组织中的鞘氨酸和鞘磷脂增加，同时复杂的鞘脂减少，这被普遍认为是伏马毒素对大多数物种的毒性作用机制。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有伏马毒素(Fumonisins)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的伏马毒素(Fumonisins)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Fumonisins antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Fumonisins in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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