计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
呕吐毒素,也被称为脱氧雪腐镰刀菌素(DON),是一种 B 型的毛霉菌,也是一种环氧树脂类物质。这种霉菌毒素主要出现在小麦、大麦、燕麦、黑麦和玉米等谷物中,在水稻、高粱和三叶草中较少出现。脱氧雪腐镰刀菌烯醇的发生主要与镰刀菌(Gibberella zeae)和镰刀菌(F. culmorum)有关, 两者都是重要的植物病原体,在小麦中引起镰刀菌头瘟,在玉米中引起镰刀菌或镰刀菌穗瘟。镰刀菌头瘟的发生与开花(花期)时的湿度密切相关,降雨的时间而不是数量是最关键的因素。然而,由于毒素的浸出,临近收获时水分的增加与小麦籽粒中呕吐毒素的含量较低有关。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有呕吐毒素(Vomitoxin)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的呕吐毒素(Vomitoxin)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Votoxin antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Votoxin in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.