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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
四环素(Tetracycline)是四环素类药物中的一种口服抗生素,用于治疗多种感 染,包括痤疮、霍乱、布鲁氏菌病、鼠疫、疟疾和梅毒。常见的副作用包括 呕吐、腹泻、皮疹和食欲不振。怀孕期间使用可能会对胎儿造成伤害。它通 过抑制细菌的蛋白质合成而发挥作用。Tetracycline 通过阻断带电 tRNA 在 P 位点肽链上的附着来抑制蛋白质合成。四环素阻断 A 位点,因此氨基酸之间不会形成氢键。四环素与微生物核糖体的 30S 和 50S 亚基结合。因此,它阻止了肽链的形成。即使停药,该作用通常也不是抑制性的和不可逆的。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有四环素(Tetracycline)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的四环素(Tetracycline)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Tetracycline antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Tetracycline in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.