计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
妥布霉素是一种源自特尼布拉里链霉菌的氨基糖苷类抗生素,用于治疗各种 类型的细菌感染,尤其是革兰氏阴性菌感染。它对假单胞菌特别有效。它于 1965 年获得专利,并于 1974 年批准用于医疗用途。它在世界卫生组织的基 本药物清单上。妥布霉素通过与细菌 30S 和 50S 核糖体上的一个位点结合而起作用,从而阻止 70S 复合物的形成。因此,mRNA 无法翻译成蛋白质,细胞死亡随之而来。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有妥布霉素(Tobramycin)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的妥布霉素(Tobramycin)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Tobramycin antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Tobramycin in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.