计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
庆大霉素是为数不多的热稳定性的抗生素,因而广泛应用于培养基配置。中国独立自主研制成功的广谱抗生素,是新中国成立以来的伟大科技成果之一。 它开始研制于 1967 年,成功鉴定在 1969 年底,取名“庆大霉素”,意指庆祝“九大”以及庆祝工人阶级的伟大。庆大霉素[Gentacin,正泰霉素(gentamycin)]系从放线菌科单孢子属发酵培养液中提得,系碱性化合物, 是常用的氨基糖苷类抗生素,主要用于治疗细菌感染,尤其是革兰氏阴性菌引起的感染。它能与细菌核糖体 30s 亚基结合,阻断细菌蛋白质合成。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有庆大霉素(Gentamicin)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的庆大霉素(Gentamicin)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Gentacin antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Gentacin in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.