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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
新霉素(Neomycin)是一种氨基糖苷类抗生素，对革兰氏阴性需氧杆菌和一些 尚未产生耐药性的厌氧杆菌具有杀菌活性。它对革兰氏阳性杆菌和厌氧革兰 氏阴性杆菌一般没有效果。新霉素有口服和外用制剂，包括药膏、软膏和眼 药水。它含有两个或多个由糖苷键连接的氨基糖。新霉素通常作为局部制剂使用，例如 Neosporin（新霉素/多粘菌素 B/杆菌肽）。新霉素不能从胃肠道吸收，已被用作肝性脑病和高胆固醇血症的预防措施。通过杀死肠道中的细菌，新霉素保持低氨水平并预防肝性脑病。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有新霉素(Neomycin)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的新霉素(Neomycin)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Neomycin antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Neomycin in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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