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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
链霉素(Streptomycin)是一种抗生素药物，用于治疗多种细菌感染，包括肺结核、禽分枝杆菌复合体、心内膜炎、布鲁氏菌病、伯克氏菌感染、鼠疫、土拉菌病和鼠咬热。常见的副作用包括眩晕、呕吐、面部麻木、发烧和皮疹。怀孕期间使用可能会导致发育中的婴儿永久性耳聋。链霉素是一种氨基糖苷类药物。它的作用原理是阻断 30S 核糖体亚基制造蛋白质的能力，从而导致细菌死亡。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有链霉素(Streptomycin)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的链霉素(Streptomycin)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Streptomycin antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Streptomycin in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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