计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
皮质酮,又称 17-脱氧皮质醇和 11β,21-二羟基黄体酮,是一种 21 碳的皮质 类固醇激素,在肾上腺皮质中产生。除了 17α-羟化酶缺乏导致的非常罕见的 先天性肾上腺增生症外,它对人类的重要性不大。在许多物种中,包括两栖 动物、爬行动物、啮齿动物和鸟类,皮质酮是一种主要的糖皮质激素,参与 调节能量、免疫反应和压力反应。皮质酮通过醛固酮合成酶转化为醛固酮, 醛固酮合成酶仅在肾小球细胞的线粒体中发现。肾小球细胞存在于肾小球区, 它是肾上腺皮质内分泌细胞的最表层区域。皮质酮是矿物皮质激素醛固酮的 前体分子,是体内钠和钾水平的主要平衡调节剂之一。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有鸡皮质酮/肾上腺酮(CORT)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的鸡皮质酮/肾上腺酮(CORT)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Chicken CORT antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Chicken CORT in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.