BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
黄曲霉毒素(AFT)是各种有毒的致癌物和诱变物，由某些霉菌，特别是曲霉菌种类产生。黄曲霉毒素及其产生菌在自然界中分布广泛，有些菌株产生不止一种类型的黄曲霉毒素，但在黄曲霉中也有不产生任何类型黄曲霉毒素的菌株。谷物和油料作物的种子及加工产品、干鲜果品、调味品、烟草、乳及乳制品、肉类、鱼虾类和动物饲料中均能检出黄曲霉素，花生和玉米最容易污染。AF 能通过食料转移到动物的乳汁、肝、肾和肌肉组织中积留。AF 属于超剧毒物质，其中 B1 是目前已知致癌物质中致癌性最强烈的，能诱发动物肝癌，对某些动物能引起急性中毒致死。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有黄曲霉毒素(AFT)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的黄曲霉毒素(AFT)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Aflatoxin (AFT) antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Aflatoxin (AFT) in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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