计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
5-羟色胺是一种吲哚衍生物,简称 5-HT,化学式为 C10H12N2O,分子量为 176.22。5-羟色胺最早是从血清中发现的,又名血清素,广泛存在于哺乳动物组织中,特别在大脑皮层质及神经突触内含量很高,它也是一种抑制性神经递质。5-羟色胺能与酸作用生成结晶盐。5-羟色胺在脑组织中的浓度较高,它是调节神经活动的一种重要物质。有些机体组织当受到某些药物作用时,可以释放出 5-羟色胺,例如一个利血平分子可以使受作用的组织释放出几百个 5-羟色胺分子,因而产生利血平的一系列生理作用。作为自体活性物质,约 90%合成和分布于肠嗜铬细胞,通常与 ATP 等物质一起储存于细胞颗粒内。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠5羟色胺(5-HT)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠5羟色胺(5-HT)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat 5-HT antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat 5-HT in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.