BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
促甲状腺素释放激素(TRH)是一种由下丘脑的神经元产生的促下丘脑激素，可刺激垂体前叶释放促甲状腺激素（TSH）和催乳素。它被翻译成242个氨基酸的前体多肽，其中包含6份序列-Gln-His-Pro-Gly-，两侧是Lys-Arg或Arg-Arg序列。室旁核的TRH合成神经元投射到正中突起外层的内侧部分。在正中隆起分泌后，它通过下丘脑门系统到达垂体前叶，在那里与TRH受体结合，刺激甲状腺刺激素和泌乳素的释放。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠促甲状腺素释放激素(TRH)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠促甲状腺素释放激素(TRH)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat TRH antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat TRH in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
危险品化学品经营许可证（带存储）