计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
叶酸(Folic Acid)是一种水溶性维生素。因绿叶中含量十分丰富而得名,又名 蝶酰谷氨酸。在自然界中有几种存在形式,其母体化合物是由蝶啶、对氨基 苯甲酸和谷氨酸 3 种成分结合而成。它在体内有主动吸收和扩散被动吸收两 种方式,吸收部位主要在小肠上部。它在蛋白质合成及细胞分裂与生长过程 中具有重要作用,对正常红细胞的形成有促进作用。缺乏时可致红细胞中血 红蛋白生成减少、细胞成熟受阻,导致巨幼红细胞性贫血。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠叶酸(Folic Acid)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠叶酸(Folic Acid)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat Folic Acid antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat Folic Acid in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.