计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
KISS1 转移抑制基因(KISS1),是由 KISS1 基因编码的蛋白质。它是 G 蛋 白偶联受体 GPR54 的配体。它最初被鉴定为具有抑制黑素瘤和乳腺癌转移 能力的转移抑制基因。Kisspeptin-GPR54 信号传导在青春期启动促性腺激 素释放激素的分泌中具有重要作用,其程度是正在进行的研究领域。它及其 受体存在于肾脏的各个部位,包括集合管、血管平滑肌和肾小管细胞。它在 低促性腺激素性腺功能减退症中起作用,它刺激参与促性腺激素释放激素 (GnRH)释放的神经元,并可能对黄体生成素(LH)和卵泡刺激素(FSH) 的释放产生一些影响。它最显着地表达在下丘脑中,但也存在于大脑的其他区域。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠吻素1(KISS1)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠吻素1(KISS1)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat KISS1 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat KISS1 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.